Single-Stranded DNA Recognition over Fluorescent Gold-Aryl Nanoparticles

Fluorescence labeling of gold-aryl nanoparticles, AuNPs-COOH, was achieved by the covalent derivatization with dansyl chloride (DNS-Cl) reagent (5-naphthalene-1-sulfonyl chloride) for potential ssDNA recognition. The fluorescent gold nanoparticles AuNPs-C6H4-4-COO-dansyl (AuNPs-DNS) spherical shape and a size 19.3 ± 8.3 nm were synthesized in carbonate-bicarbonate buffer (pH = 10.6) at 37 °C. fluorescence emission 475 acquired using spectroscopy investigated time-resolved photoluminescence. conjugation to AuNPs-DNS freeze-thaw salt-aging methods confirmed quenching, gel electrophoresis separation, lifetime decrease. Conjugated method more efficient than method. purity upon measured optical density, obtained A260/A280 ratio range 1.7–2.0. This research can be applied other nucleotide recognition theranostics.